ASTM D6974-2009 液体燃料中能活细菌和真菌计数的实施规程.过滤和培养程序

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【英文标准名称】:StandardPracticeforEnumerationofViableBacteriaandFungiinLiquidFuels8212;FiltrationandCultureProcedures
【原文标准名称】:液体燃料中能活细菌和真菌计数的实施规程.过滤和培养程序
【标准号】:ASTMD6974-2009
【标准状态】:现行
【国别】:美国
【发布日期】:2009
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D02.14
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:
【英文主题词】:bacteria;biodegradation;biodeterioration;colonyformingunit;enumeration;fuelmicrobiology;fungi;membranefiltertechnique;viablecount;Bacteria/bacterialcontrol--pretroleumproducts;Biodegradation;Biodeterioration;Colonyformingunits`;Fuel
【摘要】:Biodeteriogenicmicrobesinfectingfuelsystemstypicallyaremostabundantwithinslimeaccumulationsonsystemsurfacesoratthefuel-waterinterface(GuideD6469).However,itisoftenimpracticaltoobtainsamplesfromtheselocationswithinfuelsystems.Althoughthenumbersofviablebacteriaandfungirecoveredfromfuel-phasesamplesarelikelytobeseveralordersofmagnitudesmallerthanthosefoundinwater-phasesamples,fuel-phaseorganismsareoftenthemostreadilyavailableindicatorsoffuelandfuelsystemmicrobialcontamination.GrowthMediumSelectivity8212;GuideE1326discussesthelimitationsofgrowthmediumselection.Anymediumselectedwillfavorcolonyformationbysomespeciesandsuppresscolonyformationbyothers.Asnotedin6.3,physical,chemicalandphysiologicalvariablescanaffectviablecellenumerationtestresults.TestMethodD7463providesanon-culturemeansofquantifyingmicrobialbiomassinfuelsandfuelassociatedwater.Sinceawiderangeofsamplesizes,ordilutionsthereof,canbeanalyzedbythemembranefiltertechnique(TestMethodsD5259andF1094),thetestsensitivitycanbeadjustedforthepopulationdensityexpectedinthesample.Enumerationdatashouldbeusedaspartofdiagnosticeffortsorroutineconditionmonitoringprograms.Enumerationdatashouldnotbeusedasfuelqualitycriteria.1.1Thispracticecoversamembranefilter(MF)procedureforthedetectionandenumerationofHeterotrophicbacteria(HPC)andfungiinliquidfuelswithkinematicviscositiesx2264;24mm2x00B7;s-1atambienttemperature.1.2ThisquantitativepracticeisdrawnlargelyfromIPMethod385andTestMethodD5259.1.3Thistestmaybeperformedeitherinthefieldorinthelaboratory.1.4Theabilityofindividualmicrobestoformcoloniesonspecificgrowthmediadependsonthetaxonomyandphysiologicalstateofthemicrobestobeenumerated,thechemistryofthegrowthmedium,andincubationconditions.Consequently,testresultsshouldnotbeinterpretedasabsolutevalues.Rathertheyshouldbeusedaspartofadiagnosticorconditionmonitoringeffortthatincludesothertestparameters,inaccordancewithGuideD6469.1.5Thispracticeoffersalternativeoptionsfordeliveringfuelsamplemicrobestothefiltermembrane,volumesordilutionsfiltered,growthmediausedtocultivatefuel-bornemicrobes,andincubationtemperatures.Thisflexibilityisofferedtofacilitatediagnosticefforts.Whenthispracticeisusedaspartofaconditionmonitoringprogram,asingleprocedureshouldbeusedconsistently.1.6ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.7Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:E31
【国际标准分类号】:75_160_20
【页数】:5P.;A4
【正文语种】:英语